Journal: bioRxiv

Article Title: SFPQ-TFE3 gene fusion reciprocally regulates mTORC1 activity and induces lineage plasticity in a novel mouse model of renal tumorigenesis

doi: 10.1101/2024.11.21.624702

Figure Lengend Snippet: (A) Dual IHC for PAX8 (brown) and GPNMB (teal) in tamoxifen-injected, SFPQ-TFE3 LSL ; Pax8-CreERT transgenic mice and age-matched, littermate controls, at 2 weeks following injection of tamoxifen. Scale bar = 100 µm. (B) H&E (top row) and dual PAX8/GPNMB IHC staining (bottom row) of kidneys from representative tamoxifen-injected, SFPQ-TFE3 LSL ; Pax8-CreERT transgenic mice and age-matched, littermate controls, at 3.5 months following injection of tamoxifen. Scale bar= 100 µm. (C) H&E (top row), dual PAX8/GPNMB IHC staining (middle row) and PAX2 IHC staining (bottom row) of kidneys from representative age-matched, control and SFPQ-TFE3 LSL ; Ksp-Cre transgenic mice at post-natal day 15. Scale bar = 100 µm. (D) H&E (top row), dual PAX8/GPNMB IHC staining (middle row) and PAX2 IHC staining (bottom row) of kidneys from representative age-matched, control and PRCC-TFE3 LSL ; Ksp-Cre transgenic mice at 7 to 9 months. (E) Digital quantification of mean nuclear PAX8 H-scores in GPNMB-cells (blue) and GPNMB+ cells (red) at day 1 and day 15 (Fix Figure), from experiments in C , as depicted by scatter plots, with the central line denoting the median. The number of biological replicates analyzed for both, GPNMB – and + cells were n=11 (day 1) and n=9 (day 15). Statistical analyses were performed using two-tailed Mann-Whitney test. (F) Digital quantification of mean nuclear PAX8 H-scores in GPNMB-cells (blue) and GPNMB+ cells (red) at 4 months and 7 months, from experiments in D , as depicted by scatter plots, with the central line denoting the median. The number of biological replicates analyzed for both, GPNMB – and + cells were n=9 at 4 and 7 months. Statistical analyses were performed using two-tailed Mann-Whitney test. (G) Gene Set Enrichment Analysis (GSEA) comparing 15-day STK (top panels), 3.5-month tamoxifen-treated, STP (middle panels) and 7-month PTK (bottom panels), transgenic kidneys and their controls, for genes differentially expressed in renal TSC-related PEComas from . (H) Immunoblotting of lysates from primary renal tubular epithelial cells from SFPQ-TFE3 LSL transgenic mice treated with control or Cre-recombinase expressing adenovirus in vitro for the indicated markers. Source data are provided as a Source data file.

Article Snippet: 2) Mice hemizygous for the Ksp -Cre recombinase knockin gene (Strain Number: 012237) (The Jackson Laboratory).

Techniques: Injection, Transgenic Assay, Immunohistochemistry, Control, Two Tailed Test, MANN-WHITNEY, Western Blot, Expressing, In Vitro

Journal: eNeuro

Article Title: Cell-Specific Single Viral Vector CRISPR/Cas9 Editing and Genetically Encoded Tool Delivery in the Central and Peripheral Nervous Systems

doi: 10.1523/ENEURO.0438-23.2024

Figure Lengend Snippet: Material availability

Article Snippet: Strain, strain background (PV-IRES-Cre, C57Bl/6) , Pvalbtm1(cre)Arbr , Jackson Laboratory; PMID 15836427 ( ) , Jackson Laboratory #017320 , Mouse line with Cre recombinase knocked in to the PV gene locus.

Techniques: Recombinant, Plasmid Preparation, Cloning, Control, Expressing, Sequencing, Software, CRISPR